We recently launched a new product, Universal Cloning Kit, which is primarily for cloning PCR products into any vector of your choice. This cloning kit is similar to Clontech’s In-Fusion Cloning System and Life Technologies’ GeneArt Seamless Cloning and Assembly Kit, but has a higher cloning efficiency. The cloning reaction is very robust and easy to carry out. We found that many DNA constructs that were failed to be constructed with restriction enzymes and T4 ligase were succeeded by just one try with this kit. For more information please go to Universal Cloning Kit .
Screen of cDNA Libraries
We perform cDNA library screen for researchers to discover their proteins of interest. Our screen methods are based on either proteins or cellular functions. These methods have been used to make many break through discoveries.
Protein Production and Purification
We use mammalian cells to produce biologically active proteins with non-infectious vaccinia expression systems. Our services satisfy your needs for a single protein as well as high throughput production of tens and hundreds of different proteins.
Making Stable Expression Cell Lines
We provide services to make stably transformed cell lines. One of the methods we are using is to deliver a foreign gene into the target cells with lentivral vectors. Lentiviral protein-mediated integration renders long term expression of foreign genes in the target cells.
Construction of cDNA Libraries
We have a proprietary technology to construct cDNA libraries. Using this technology, we are able to directionally clone cDNA into almost any types of expression vectors without cutting the cDNA with restriction enzymes. Therefore, the cDNA libraries we constructed have higher complexities and more full length clones than the others'.
Synthesis of RNA
We provide services to synthesize a variety of RNA by in vitro transcription. The mRNA that we synthesize is capped and has a long poly(A) tail of more than 50 adenine nucleotides. It is particularly suitable for in vivo protein synthesis.
Antibody Cloning and Expression
We have a proprietary technology to clone the heavy (H) and light (L) chain cDNA of antibodies from hybridoma cells. With this technology, we are able to clone H and L cDNA even from low antibody-expression hybridoma cells.